Non-escaping frost tolerant QTL linked genetic loci at reproductive stage in six wheat DH populations

Authors

Jingjuan Zhang, Murdoch University
MD Shahidul Islam, Murdoch University
Yun Zhao, Murdoch University
Masood Anwar, Murdoch University
Zaid Alhabbar, Murdoch University
Maoyun She, Murdoch University
Rongchang Yang, Murdoch University
Angela Juhasz, Murdoch University
Guixiang Tang, Murdoch University
Jiansheng Cheng, Murdoch University
Darshan L. Sharma, Department of Primary Industries and Regional DevelopmentFollow
Nathan Height, Department of Primary Industries and Regional DevelopmentFollow
Ben Biddulph, Department of Primary Industries and Regional DevelopmentFollow
Hang Liu, Murdoch University
Yanjie Jiang, Murdoch University
Shengnan Zhai, Murdoch University
Xin Hu, Murdoch University
Junkang Rong, Murdoch University
Yingquan Zhang, Murdoch University
Yebo Qin, Murdoch University
Qier Liu, Murdoch University
Zitong Yu, Murdoch University
Yujuan Zhang, Murdoch University
Sadegh Balotf, Murdoch University
Mirza Dowla, Murdoch University
Sonia Afrin, Murdoch University
Nandita Roy, Murdoch University
Md Resad Mallik, Murdoch University
Md Atik Us Saieed, Murdoch University
Shanjida Rahman, Murdoch University
Nigarin Sultana, Murdoch University
Sarah al-Sheikh Ahmed, Murdoch University
Chris Florides, Murdoch University
Kefei Chen, Curtin University of Technology
Meigin Lu, Australian Grain Technologies
Jorge E. Mayer, Ag RD&IP Consult P/L
Wujun Ma, Murdoch University

Document Type

Article

Publication Date

2-1-2022

Journal Title

The Crop Journal

ISSN

Print: 2095-5421; Electronic: 2214-5141

Keywords

Anthesis-related genes; Wheat DH populations; Reproductive stage; Non-escaping frost tolerance; QTL

Disciplines

Agricultural Science | Agriculture | Agronomy and Crop Sciences | Plant Breeding and Genetics

Abstract

Reproductive stage frost poses a major constraint for wheat production in countries such as Australia. However, little progress has been made in identifying key genes to overcome the constraint. In the present study, a severe frost event hit two large-scale field trials consisting of six doubled haploid (DH) wheat populations at reproductive stage (young microspore stage) in Western Australia, leading to the identification of 30 robust frost QTL on 17 chromosomes. The major 18 QTL with the phenotype variation over 9.5% were located on 13 chromosomes including 2A, 2B, 2D, 3A, 4A, 4B, 4D, 5A, 5D, 6D, 7A, 7B and 7D. Most frost QTL were closely linked to the QTL of anthesis, maturity, Zadok stages as well as linked to anthesis related genes. Out of those, six QTL were repetitively detected on the homologous regions on 2B, 4B, 4D, 5A, 5D, 7A in more than two populations. Results showed that the frost damage is associated with alleles of Vrn-A1a, Vrn-D1a, Rht-B1b, Rht-D1b, and the high copy number of Ppd-B1. However, anthesis QTL and anthesis related genes of Vrn-B1a and TaFT3-1B on chromosomes 5B and 1B did not lead to frost damage, indicating that these early-flowering phenotype related genes are compatible with frost tolerance and thus can be utilised in breeding. Our results also indicate that wild-type alleles Rht-B1a and Rht-D1a can be used when breeding for frost-tolerant varieties without delaying flowering time.

Recommended Citation

Zhang, J, Islam, M, Zhao, Y, Anwar, M, Alhabbar, Z, She, M, Yang, R, Juhasz, A, Tang, G, Cheng, J, Sharma, D L, Height, N, Biddulph, B, Liu, H, Jiang, Y, Zhai, S, Hu, X, Rong, J, Zhang, Y, Qin, Y, Liu, Q, Yu, Z, Zhang, Y, Balotf, S, Dowla, M, Afrin, S, Roy, N, Mallik, M R, Saieed, M A, Rahman, S, Sultana, N, Ahmed, S a, Florides, C, Chen, K, Lu, M, Mayer, J E, and Ma, W. (2022), Non-escaping frost tolerant QTL linked genetic loci at reproductive stage in six wheat DH populations. The Crop Journal, 10 (1), 147-165.
https://library.dpird.wa.gov.au/fc_researchart/18