Assessing the activity of antimicrobial peptides against common marine bacteria located in Rotifer (Brachionus plicatilis) cultures

Authors

Chelsea Woods, Department of Primary Industries and Regional Development, Fremantle, Perth, Western AustraliaFollow
Lindsey Woolley, Department of Primary Industries and Regional Development, Fremantle, Perth, Western AustraliaFollow
Gavin Partridge, Department of Primary Industries and Regional Development, Fremantle, Perth, Western Australia
Mengqi Chen, Department of Primary Industries and Regional Development, Fremantle, Perth, Western AustraliaFollow
Evan F. Haney, Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
Robert E.W. Hancock, Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada
Nicky Buller, Department of Primary Industries and Regional Development, Fremantle, Perth, Western AustraliaFollow
Andrew Currie, Murdoch University, Perth, Western Australia

Document Type

Article

Publication Date

5-25-2022

Journal Title

Probiotics and Antimicrobial Proteins

ISSN

ISSN 1867-1306 eISSN 1867-1314

Keywords

Rotifers, Marine fish larvae, Antimicrobial peptides (AMPs), Artificial seawater, Bacteria, Probiotics, Antibacterial activity, Minimum inhibitory concentration, Brachionus plicatilis

Disciplines

Aquaculture and Fisheries | Bacteriology | Environmental Microbiology and Microbial Ecology | Molecular, Genetic, and Biochemical Nutrition | Natural Resources Management and Policy

Abstract

Rotifers are used as the first feed for marine fish larvae and are grown in large cultures that have high loads of organic matter and heterotrophic bacteria; these bacteria are passed on to the developing fish larvae and can potentially lead to bacterial infections. A modified minimum inhibitory concentration (MIC) protocol for antimicrobial peptides was used to determine the potency of ten antimicrobial peptides (AMPs) in artificial seawater relevant to a rotifer culture (salinity of 25‰) against common marine pathogens. All of the AMPs had antimicrobial activity against the bacterial isolates when the salt concentration was approximately zero. However, in high salt concentrations, the majority of the AMPs had an MIC value greater than 65 µg mL⁻¹ in artificial seawater (25‰). The only exceptions were 2009 (32.5 µg mL⁻¹) and 3002 (32.5 µg mL⁻¹) against Vibrio rotiferianus and Tenacibaculum discolor, respectively. The selected synthetic AMPs were not effective at reducing the bacterial load in brackish salt concentrations of a typical commercial rotifer culture (25‰).

Recommended Citation

Woods, C., Woolley, L., Partridge, G. et al. Assessing the Activity of Antimicrobial Peptides Against Common Marine Bacteria Located in Rotifer (Brachionus plicatilis) Cultures. Probiotics & Antimicro. Prot. 14, 620–629 (2022). https://doi.org/10.1007/s12602-022-09928-2