A novel monoclonal Perkinsus chesapeaki in vitro isolate from an Australian cockle, Anadara trapezia

Authors

Kimberly S. Reece, Virginia Institute of Marine Science
Gail P. Scott, Virginia Institute of Marine Science
Cecile Dang, Department of Primary Industries and Regional Development, Western AustraliaFollow
Christopher F. Dungan, Maryland Department of Natural Resources, Oxford

Document Type

Article

Publication Date

5-24-2017

Journal Title

Journal of Invertebrate Pathology

ISSN

0022-2011

Keywords

Perkinsus sp, monoclonal isolate, Phylogenetic analyses, Perkinsus olseni, Perkinsus chesapeaki

Disciplines

Aquaculture and Fisheries

Abstract

A monoclonal Perkinsus chesapeaki isolate was established from 1 of 10 infected Australian Anadara trapezia cockles. Morphological features were similar to those of described P. chesapeaki isolates, and also included a unique vermiform schizont cell-type. Perkinsus olseni-specific PCR primers amplified DNAs from all 10 cockles. Perkinsus chesapeaki-specific primers also amplified DNAs from 4/10 cockles, including DNA from the isolate source cockle. Three different sets of DNA sequences from the monoclonal isolate grouped with the homologous, previously deposited, P. chesapeaki sequences in phylogenetic analyses. In situ hybridization assays detected both P. chesapeaki and P. olseni cells in histological sections from the source cockle for monoclonal isolate ATCC PRA-425.

Recommended Citation

Reece, K S, Scott, G P, Dang, C, and Dungan, C F. (2017), A novel monoclonal Perkinsus chesapeaki in vitro isolate from an Australian cockle, Anadara trapezia. Journal of Invertebrate Pathology, 148, 86-93.
https://library.dpird.wa.gov.au/fr_fja/437