Fisheries Research Articles
Draft genomes of Perkinsus olseni and Perkinsus chesapeaki reveal polyploidy and regional differences in heterozygosity
Document Type
Article
Publication Date
10-2-2020
Journal Title
Genomics
ISSN
0888-7543
Keywords
Perkinsus, Genomics, Ploidy, Heterozygosity
Disciplines
Aquaculture and Fisheries
Abstract
Perkinsus spp. parasites have significant impact on aquaculture and wild mollusc populations. We sequenced the genomes of five monoclonal isolates of Perkinsus olseni and one Perkinsus chesapeaki from international sources. Sequence analysis revealed similar levels of repetitive sequence within species, a polyploid genome structure, and substantially higher heterozygosity in Oceanian-sourced isolates. We also identified tandem replication of the rRNA transcriptional unit, with high strain variation. Characterized gene content was broadly similar amongst all Perkinsus spp. but P. olseni Oceanian isolates contained an elevated number of genes compared to other P. olseni isolates and cox3 could not be identified in any Perkinsus spp. sequence. Phylogenetics and average nucleotide identity scans were consistent with all P. olseni isolates being within one species. These are the first genome sequences generated for both P. olseni and P. chesapeaki and will allow future advances in diagnostic design and population genomics of these important aquatic parasites.
Recommended Citation
Bogema, D R,
Yam, J,
Micallef, M L,
Gholipour-Kanani, H,
Go, J,
Jenkins, C,
and
Dang, C.
(2020), Draft genomes of Perkinsus olseni and Perkinsus chesapeaki reveal polyploidy and regional differences in heterozygosity. Genomics, 113 (1), 677-688.
https://library.dpird.wa.gov.au/fr_fja/427
