Proteomic profiling of developing wheat heads under water-stress

Authors

Shahidul Islam, Murdoch University
Penghao Wang, Murdoch University
Delphine Vincent, Agriculture Victoria
Javed M. Khan, Proteomics International
Angela Juhasz, Edith Cowan University
Dean A. Diepeveen, Department of Primary Industries and Regional Development, Western AustraliaFollow
Richard J. Lipscombe, Proteomics International
Wujun Ma, Murdoch University
Rudi Appels, University of Melbourne

Document Type

Article

Publication Date

7-17-2020

Journal Title

Functional and Integrative Genomics

ISSN

ISSN: 1438-793X eISSN: 1438-7948

Keywords

iTRAQ, Protein expression, Water-stress, Wheat head

Disciplines

Agricultural Science | Agronomy and Crop Sciences | Plant Breeding and Genetics

Abstract

A replicated iTRAQ (isobaric tags for relative and absolute quantification) study on developing wheat heads from two doubled haploid (DH) lines identified from a cross between cv Westonia x cv Kauz characterized the proteome changes influenced by reproductive stage water-stress. All lines were exposed to 10 days of water-stress from early booting (Zadok 40), with sample sets taken from five head developmental stages. Two sample groups (water-stressed and control) account for 120 samples that required 18 eight-plex iTRAQ runs. Based on the IWGSC RefSeq v1 wheat assembly, among the 4592 identified proteins, a total of 132 proteins showed a significant response to water-stress, including the down-regulation of a mitochondrial Rho GTPase, a regulator of intercellular fundamental biological processes (7.5 fold) and cell division protein FtsZ at anthesis (6.0 fold). Up-regulated proteins included inosine-5′-monophosphate dehydrogenase (3.83 fold) and glycerophosphodiester phosphodiesterase (4.05 fold). The Pre-FHE and FHE stages (full head emerged) of head development were differentiated by 391 proteins and 270 proteins differentiated the FHE and Post-FHE stages. Water-stress during meiosis affected seed setting with 27% and 6% reduction in the progeny DH105 and DH299 respectively. Among the 77 proteins that differentiated between the two DH lines, 7 proteins were significantly influenced by water-stress and correlated with the seed set phenotype response of the DH lines to water-stress (e.g. the up-regulation of a subtilisin-like protease in DH 299 relative to DH 105). This study provided unique insights into the biological changes in developing wheat head that occur during water-stress.

Recommended Citation

Islam, S., Wang, P., Vincent, D. et al. Proteomic profiling of developing wheat heads under water-stress. Funct Integr Genomics 20, 695–710 (2020). https://doi.org/10.1007/s10142-020-00746-9