Burkholderia sprentiae sp. nov., isolated from Lebeckia ambigua root nodules
Document Type
Article
Publication Date
11-1-2013
Journal Title
International Journal of Systematic and Evolutionary Microbiology
ISSN
ISSN: 1466-5026 E-ISSN: 1466-5034
Keywords
bacteria, root nodules, phylogenetic trees, Burkholderia
Disciplines
Agronomy and Crop Sciences
Abstract
Seven Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia , with the representative strain WSM5005T being most closely related to Burkholderia tuberum (98.08 % sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia . Results of DNA–DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest species of the genus Burkholderia with a validly published name. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (type strain WSM5005T = LMG 27175T = HAMBI 3357T) is proposed.
Recommended Citation
De Meyer, S E,
Cnockaert, M,
Ardley, J K,
Maker, G,
Yates, R J,
Howieson, J G,
and
Vandamme, P.
(2013), Burkholderia sprentiae sp. nov., isolated from Lebeckia ambigua root nodules. International Journal of Systematic and Evolutionary Microbiology, 63 (11).
https://library.dpird.wa.gov.au/fc_researchart/240
