A porous agar medium for improving the growth of plants under sterile conditions
Document Type
Article
Publication Date
5-1988
Journal Title
Plant and Soil
ISSN
Print: 0032-079X Electronic: 1573-5036
Keywords
gaseous diffusion, root growth, sterile agar medium, Trifolium subterraneum
Disciplines
Agronomy and Crop Sciences
Abstract
Porous nutrient agar was prepared under sterile conditions by drawing molten 3.5% (w/v) nutrient agar into a plastic syringe, allowing it to set, extruding it into a test tube and giving the tube a firm flick. Simple colorimetric tests showed that gaseous diffusion was substantially faster through 3.5% (w/v) porous agar than through the 1% (w/v) non-porous agar frequently used for growing plants under sterile conditions. Root systems of Trifolium subterraneum grew 80–90% larger in porous than in non-porous agar.
Recommended Citation
Barrett-Lennard, E.G., Dracup, M. A porous agar medium for improving the growth of plants under sterile conditions. Plant Soil 108, 294–298 (1988). https://doi.org/10.1007/BF02375663
