Biosecurity Research Articles
Quantitative disease resistance assessment by real-time PCR using the Stagonospora nodorum-wheat pathosystem as a model
Document Type
Article
Publication Date
2-13-2008
Journal Title
Plant Pathology
ISSN
Print: 0032-0862 Electronic: 1365-3059
Keywords
breeding for disease resistance, glume blotch of wheat, stagonospora leaf blotch, Triticum aestivum
Disciplines
Agronomy and Crop Sciences | Biosecurity | Plant Breeding and Genetics
Abstract
Measuring disease resistance accurately and reproducibly is a key requirement for the introgression of partial resistance genes into breeding lines. Here, a qPCR protocol is used to measure fungal biomass, using the wheat-Stagonospora nodorum pathosystem as a model. Seven cultivars of differing reported resistance levels were used. Fungal biomass taken at 220°C thermal days after inoculation accurately predicts the final grain weight loss. It is concluded that a test based on qPCR methods is specific, quantitative, rapid and objective. Such tests could provide useful and economic tools in the development of robustly resistant crop cultivars.
Recommended Citation
Oliver, R P,
Rybak, K,
Shankar, M,
Loughman, R,
Harry, N,
and
Solomon, P S.
(2008), Quantitative disease resistance assessment by real-time PCR using the Stagonospora nodorum-wheat pathosystem as a model. Plant Pathology, 57 (3), 527-532.
https://library.dpird.wa.gov.au/bs_research/91
