Biosecurity Research Articles
Identification and characterization of stripe rust resistance gene Yr34 in common wheat
Document Type
Article
Publication Date
1-25-2006
Journal Title
Theoretical and Applied Genetics
ISSN
Print: 0040-5752 Electronic: 1432-2242
Disciplines
Agronomy and Crop Sciences | Biosecurity | Plant Breeding and Genetics
Abstract
An uncharacterized source of seedling resistance to Puccinia striiformis f.sp. tritici was identified in an advanced wheat breeding line WAWHT2046. Genetic analysis based on a WAWHT2046/Carnamah-derived double haploid (DH) population demonstrated monogenic inheritance of seedling stripe rust resistance in WAWHT2046. The gene controlling stripe rust resistance in line WAWHT2046 was tentatively designated YrWA. The chromosome 5AL located awn inhibitor gene B1, possessed by WAWHT2046, also showed monogenic inheritance when the DH population was scored for the presence and absence of awns. Joint segregation analysis at the B1 and YrWA loci indicated genetic linkage between the two loci. A recombination value of 12.2 cM was computed using Mapmanager. This association located YrWA in the chromosome arm 5AL. Molecular mapping using microsatellite markers placed YrWA distal to B1. All molecular markers mapped proximal to the awn inhibitor locus B1. As no other stripe rust resistance gene is reported to be located in the chromosome arm 5AL, YrWA was permanently designated as Yr34. Yr34 produced an intermediate (23C) seedling infection type and expressed very low stripe rust response (10R-MR) on adult plants in the field, similar to the resistance gene Yr17. In addition to Yr34, this mapping population segregated for three genetically independent adult plant stripe rust resistance genes. The detection of DH lines with completely susceptible response, higher than that shown by the Yr34-lacking parent Carnamah, suggested that both parents contributed adult plant resistance. The use of WAWHT2046 as a parent in breeding programs would also contribute APR in addition to Yr34.
Recommended Citation
Bariana, H.S., Parry, N., Barclay, I.R. et al. Identification and characterization of stripe rust resistance gene Yr34 in common wheat. Theor Appl Genet 112, 1143–1148 (2006). https://doi.org/10.1007/s00122-006-0216-3
