Detection and quantification of Sclerotinia sclerotiorum infestation of canola petals using a clearing and staining technique

Authors

Kithsiri W. Jayasena, Department of Primary Industries and Regional Development, Western AustraliaFollow
Andrew van Burgel, Department of Primary Industries and Regional DevelopmentFollow
Jean Galloway, Department of Primary Industries and Regional Development, Western AustraliaFollow
Geoff J. Thomas, Department of Primary Industries and Regional Development, Western AustraliaFollow

Document Type

Article

Publication Date

10-12-2022

Journal Title

Canadian Journal of Plant Pathology

ISSN

Print: 0706-0661 Electronic: 1715-2992

Keywords

Brassica napus; light microscopy; sclerotinia stem rot

Disciplines

Agronomy and Crop Sciences

Abstract

Petal testing can be used to determine the presence of Sclerotinia sclerotiorum inoculum during canola flowering as an indicator of disease risk and the potential need for fungicide application. Traditionally, the culture plate method has been used to test petals, but this takes up to 7 days to obtain results, which is a drawback in terms of making timely fungicide use decisions. Therefore, the availability of a simple, faster detection technique for S. sclerotiorum infected canola petals would be an advantage. We have documented and compared a canola petal clearing and staining technique with the standard culture plate method to study sclerotinia ascospore infestation. The staining technique was found to be a rapid, simple and reliable method compared to the traditional culture plate system.

Recommended Citation

Jayasena, K W, van Burgel, A, Galloway, J, and Thomas, G J. (2022), Detection and quantification of Sclerotinia sclerotiorum infestation of canola petals using a clearing and staining technique. Canadian Journal of Plant Pathology, 45 (1), 70-75.
https://library.dpird.wa.gov.au/fc_researchart/68