Genome sequence of Rhizobium leguminosarum bv trifolii strain WSM1689, the microsymbiont of the one flowered clover Trifolium uniflorum

Authors

Jason Terpolilli, Murdoch University
Tian Rui, Murdoch University
Ronald J. Yates, Department of Primary Industries and Regional Development, Western AustraliaFollow
John Gregory Howieson, Murdoch UniversityFollow
Philip Poole, Murdoch University
Christine Munk, Los Alamos National Laboratory, USA
Roxanne Tapia, DOE Joint Genome Institute, USA
Cliff Han, Los Alamos National Laboratory, USA
Victor Markowitz, Lawrence Berkeley National Laboratory, USA
Reddy Tatiparthi, DOE Joint Genome Institute, USA
Konstantinos Mavromatis, DOE Joint Genome Institute, USA
Natalia Ivanova, DOE Joint Genome Institute, USA
Amrita Pati, DOE Joint Genome Institute, USA
Lynne Goodwin, Los Alamos National Laboratory, USA
Tanja Woyke, DOE Joint Genome Institute, USA
Nikos Kyrpides, DOE Joint Genome Institute, USA
Wayne Reeve, Murdoch University

Document Type

Article

Publication Date

6-15-2014

Journal Title

Standards in Genomic Sciences

ISSN

1944-3277

Keywords

root-nodule bacteria, nitrogen fixation, lupin-nodulating, rhizobia, Alphaproteobacteria

Disciplines

Agronomy and Crop Sciences | Plant Breeding and Genetics

Abstract

Rhizobium leguminosarum bv. trifolii is a soil-inhabiting bacterium that has the capacity to be an effective N2-fixing microsymbiont of Trifolium (clover) species. R. leguminosarum bv. trifolii strain WSM1689 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Trifolium uniflorum collected on the edge of a valley 6 km from Eggares on the Greek Island of Naxos. Although WSM1689 is capable of highly effective N2-fixation with T. uniflorum, it is either unable to nodulate or unable to fix N2 with a wide range of both perennial and annual clovers originating from Europe, North America and Africa. WSM1689 therefore possesses a very narrow host range for effective N2 fixation and can thus play a valuable role in determining the geographic and phenological barriers to symbiotic performance in the genus Trifolium. Here we describe the features of R. leguminosarum bv. trifolii strain WSM1689, together with the complete genome sequence and its annotation. The 6,903,379 bp genome contains 6,709 protein-coding genes and 89 RNA-only encoding genes. This multipartite genome contains six distinct replicons; a chromosome of size 4,854,518 bp and five plasmids of size 667,306, 518,052, 341,391, 262,704 and 259,408 bp. This rhizobial genome is one of 20 sequenced as part of a DOE Joint Genome Institute 2010 Community Sequencing Program.

Recommended Citation

Terpolilli, J., Rui, T., Yates, R. et al. Genome sequence of Rhizobium leguminosarum bv trifolii strain WSM1689, the microsymbiont of the one flowered clover Trifolium uniflorum. Stand in Genomic Sci 9, 527–539 (2014). https://doi.org/10.4056/sigs.4988693